We established a shortened protocol for western blot analysis using intermittent microwave irradiation. With this method, the procedure is completed within 1 h after applying the primary antibody, and thus greatly saves time. This procedure appears to be applicable to any antibody based on our experience of several years.
CITATION STYLE
Liu, Y. T., & Toyokuni, S. (2009). Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis. Methods in Molecular Biology (Clifton, N.J.), 536, 307–312. https://doi.org/10.1007/978-1-59745-542-8_33
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