Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis.

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Abstract

We established a shortened protocol for western blot analysis using intermittent microwave irradiation. With this method, the procedure is completed within 1 h after applying the primary antibody, and thus greatly saves time. This procedure appears to be applicable to any antibody based on our experience of several years.

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Liu, Y. T., & Toyokuni, S. (2009). Intermittent microwave irradiation facilitates antigen-antibody reaction in Western blot analysis. Methods in Molecular Biology (Clifton, N.J.), 536, 307–312. https://doi.org/10.1007/978-1-59745-542-8_33

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