Site-directed mutagenesis has been widely used for the substitution, addition or deletion of nucleotide residues in a defined DNA sequence. QuikChange™ site-directed mutagenesis and its related protocols have been widely used for this purpose because of convenience and efficiency. We have recently demonstrated that the mechanism of the QuikChange™ site-directed mutagenesis process is different from that being proposed. The new mechanism promotes the use of partially overlapping primers and commercial PCR enzymes for efficient PCR and mutagenesis.
CITATION STYLE
Xia, Y., & Xun, L. (2017). Revised mechanism and improved efficiency of the quikchange site-directed mutagenesis method. In Methods in Molecular Biology (Vol. 1498, pp. 367–374). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6472-7_25
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