The present chapter aims to assess the motion and deformation index of red blood cells (RBCs) flowing through a microchannel with a hyperbolic contraction using an image analysis based method. For this purpose, a microchannel containing a hyperbolic contraction was fabricated in polydimethylsiloxane by using a soft-lithography technique and the images were captured by a standard high-speed microscopy system. An automatic image processing and analyzing method has been developed in a MATLAB environment, not only to track both healthy and exposed RBCs motion but also to measure the deformation index along the microchannel. The keyhole model has proved to be a promising technique to track automatically healthy and exposed RBCs flowing in this kind of microchannels.
CITATION STYLE
Taboada, B., Monteiro, F. C., & Lima, R. (2015). Tracking red blood cells flowing through a microchannel with a hyperbolic contraction: An automatic method. Lecture Notes in Computational Vision and Biomechanics, 19, 105–119. https://doi.org/10.1007/978-3-319-13407-9_7
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