Dissolution dynamic nuclear polarization (D-DNP) is a technique to prepare hyperpolarized nuclear spin states, yielding a signal enhancement of several orders of magnitude for liquid-state NMR. Here, we describe experimental procedures for the application of D-DNP in high-resolution NMR of biochemical compounds, to determine the time evolution of biochemical processes and intermolecular interactions.
CITATION STYLE
Kim, Y., Wang, Y., Chen, H. Y., & Hilty, C. (2018). In-vitro dissolution dynamic nuclear polarization for sensitivity enhancement of NMR with biological molecules. In Methods in Molecular Biology (Vol. 1688, pp. 155–168). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7386-6_8
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