The Development of IR Super-Resolution Microscopy for Direct Visualization of Single Living Cells

Abstract

We have developed novel IR super-resolution microscopy combining a laser fl uorescence microscope and transient fl uorescence detected IR spectroscopy and succeeded in performing IR imaging on onion (Allium cepa) root cells labeled by rhodamine-6G. We also demonstrated picosecond time-resolved IR imaging of vibrational relaxation of rhodamine-6G in onion root cells, and found an abnormally long-lived component on vibrational relaxation.