ADP-ribosylation, a posttranslational modification catalyzed by a family of enzymes known as poly(ADP-ribose) polymerases (PARPs, 17 in humans), regulates diverse cellular processes. To aid in understanding the functions of ADP-ribosylation in cells, we developed a clickable aminooxy probe, AO-alkyne, which detects ADP-ribosylation of acidic amino acids. AO-alkyne can be used to detect auto-ADP-ribosylation of PARP10 in cells following Cu-catalyzed click conjugation to an azide reporter. This method can be extended to other PARP family members that catalyze ADP-ribosylation on acidic amino acids, providing a convenient and direct readout of PARP activity in cells.
CITATION STYLE
Morgan, R. K., & Cohen, M. S. (2017). Detecting protein ADP-ribosylation using a clickable aminooxy probe. In Methods in Molecular Biology (Vol. 1608, pp. 71–77). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6993-7_6
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