Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of personalized in vitro disease models, genomic health analyses, and autologous cell therapy. Here we describe the generation of T lymphocyte-derived iPSCs from small, clinically advantageous volumes of non-mobilized peripheral blood. These T-cell derived iPSCs (''TiPS'') retain a normal karyotype and genetic identity to the donor. They share common characteristics with human embryonic stem cells (hESCs) with respect to morphology, pluripotency-associated marker expression and capacity to generate neurons, cardiomyocytes, and hematopoietic progenitor cells. Additionally, they retain their characteristic T-cell receptor (TCR) gene rearrangements, a property which could be exploited for iPSC clone tracking and T-cell development studies. Reprogramming T-cells procured in a minimally invasive manner can be used to characterize and expand donor specific iPSCs, and control their differentiation into specific lineages. © 2010 Brown et al.
CITATION STYLE
Brown, M. E., Rondon, E., Rajesh, D., Mack, A., Lewis, R., Feng, X., … Nuwaysir, E. F. (2010). Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes. PLoS ONE, 5(6). https://doi.org/10.1371/journal.pone.0011373
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