Protein modification by SUMO proteins is one of the key posttranslational modifications in eukaryotes. Here, we describe a workflow to analyze SUMO dynamics in response to different stimuli, purify SUMO conjugates, and analyze the changes in SUMOylation level in organisms, tissues, or cell culture. We present a protocol for lysis in denaturing conditions that is compatible with downstream IMAC and antibody affinity purification, followed by mass spectrometry and data analysis.
CITATION STYLE
Drabikowski, K., & Dadlez, M. (2016). Dissecting SUMO dynamics by mass spectrometry. In Methods in Molecular Biology (Vol. 1449, pp. 291–298). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3756-1_18
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