Induction of isoflavonoid and retrochalcone branches of the flavonoid pathway in cultured glycyrrhiza echinata cells treated with yeast extract

Abstract

Yeast extract-treated suspension cultures of a new cell line, AK-1, of Glycyrrhiza echinata were induced to produce an isoflavonoid phytoalexin (medicarpin) and metabolites of retrochalcone/flavone pathway (echinatin, licodione, and 7,4′-dihydroxyflavone). From these cells, putative full-length cDNAs encoding cytochrome P450s,(2S)-flavanone 2-hydroxylase and isoflavone 2′-hydroxylase, were cloned. © 1999, Taylor & Francis Group, LLC. All rights reserved.