Evaluation of clcuv tolerance in cotton (Gossypium hirsutum) varieties by DNA detection

Abstract

Cotton leaf curl virus (ClCuV) has the most damaging and rapidly evolving virus spp infecting cotton (Gossypium hirsutum L.) and make cotton leaf curl disease complex. CLCuD is caused by Bemicia tabaci transmitted monopartite begomovirus whose genome is composed of DNA-A and satellite components. Present study of cotton was designed to amplify the circular single stranded molecule of begomovirus associated with CLCuD through RCA technique. Twenty lines/varieties of cotton were evaluated for their response against cotton leaf curl virus. Total genomic DNA from these samples were isolated from cotton varieties/lines and full length viral DNA-A components from CLCuV infected cotton plants were polymerized through PCR by using universal begomo 01 and begomo 02 primers. Further insilico study of different viruses with restriction enzymes PstI and EcoRI was done. The residual viral titer which was evaluated through PCR, its value was compared with the visually observed symptoms to evaluate the plant response. All varieties showed disease symptoms of which susceptible varieties were FH-114. MNH-886, LALAZAR, MS-71, MS-289, FH-682, FH-900, Z-31, ALI AKBR-703, N-112. Moreover, moderate susceptible varieties were FH-942, FH-142, MNH-86, CM-595, HUMA-15, ALI AKBR-905. However, moderate resistant varieties were FH-941, CM-595, ALI AKBR-905, CA-12.