Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions

Abstract

DAMASCENA, N. P.; SANTOS-FILHO, M. V. C. ; DE SOUZA, G. R. B.; DA SILVA, L. A. F.; ESTEVAM, C. S.; FRANCO, A.; PARANHOS, L. R. & MUSSE, J. O. Testing the extraction of DNA from human teeth exposed to different chemical solutions. ABSTRACT: The aim of this study was to evaluate the extraction of dental DNA exposed to different chemical solutions. The sample was composed by 15 subjects, from which 5 samples of oral mucosal cells (reference population) and 15 teeth (experimental population) were collected. The experimental population was divided in three equal parts, which were exposed to different chemical solutions, namely hydrochloric acid (HCl) at 37 %, formaldehyde (CH 2 O) at 10 % and sodium hypochlorite (NaOCl) at 2.5 %. The DNA from the oral mucosa was extracted using organic method, while the dental DNA was extracted using the AFDIL method, including amplification by PCR and sequencing through capillary electrophoresis. The DNA exposed to hydrochloric acid dissolved, lacking extraction. The exposure of teeth to formaldehyde and sodium hypochlorite did not interfere in the extraction of DNA, once the amplification was visible in both experimental populations. The present outcomes demonstrated that DNA extraction may be limited under exposure to chemical solutions.