Avaliação de ionóforos pela técnica da perda do potássio celular e produção de gases in vitro

Abstract

Two studies were carried out with lactating cows as experimental units and ruminal fluid donors. The ruminal bacteria population was used to evaluate the action of increasing levels of lasalocid and monensin on resistance of intracellular potassium depletion and in vitro gas production intracellular depletion potassium (Kmax) of lasalocid was lower to ruminal bacteria population obtained from rumen of cows fed diets with monensin, soybean oil and monensin plus soybean oil (19.4 to 25.4%) when compared to cows fed with control diet (30.1%). The same occurred for intracellular depletion potassium (K max) of monensin, in which the lowest value was 6.5% to monensin plus soybean oil and the greatest was 29.5% to control. High monensin concentration (Kd= 2.3 μM) and low lasalocid concentration (Kd= 0.2 μM) were necessary to cause half of maximum potassium depletion in ruminal bacteria population from cows fed diet with monensin. The ruminal bacteria population from cows feed diet with monensin were sensible to lasalocid. In vitro gas production showed the lowest volume when diets were incubated with propolis (12,9ml/100g of DM).