Zebrafish serotonin-N-acetyltransferase-2 gene regulation: Pineal-restrictive downstream module contains a functional E-box and three photoreceptor conserved elements

Abstract

Pineal function is defined by a set of very narrowly expressed genes that encode proteins required for photoperiodic transductlon and rhythmic melatonin secretion. One of these proteins is serotonin N-acetyltransferase (arylalkylamlne N-acetyltransferase, AANAT), which controls the daily rhythm in melatonin production. Here, pineal-specific expression of the zebrafish aanat-2 (zfaanat-2) was studied using in vivo transient expression analyses of promoter-reporter constructs; this revealed that specificity is determined by two regions located 12 kb away from each other. One is the 5′-flanking region, and the other is a 257-bp sequence, located 6 kb downstream of the transcribed region. This 3′-sequence, designated pineal-restrictive downstream module (PRDM), has a dual function: enhancement of pineal expression and inhibition of extrapineal expression. The former is an autonomic property of PRDM whereas the later function requires interaction with the upstream regulatory region of zfaanat-2. Functional analyses of the PRDM sequence revealed that three photoreceptor conserved elements (TAATC) and a single perfect E-box (CACGTG) are crucial for the dual function of PRDM. These results indicate that pineal specificity of zfaanat-2 is determined by the dual functionality of the PRDM and the interaction between upstream regulatory region and downstream photoreceptor conserved elements and E-box element.