Extraction, separation and purification of acidic polysaccharide from Morchella esculenta by high voltage pulsed electric field

Abstract

The intracellular acidic polysaccharide from Morchella esculenta mycelium produced by submerged fermentation is extracted and separated. In the process of extracting the polysaccharide by the high voltage pulsed electric field (HVPEF), heat is not generated and the structure of the polysaccharide is not destroyed. The response surface method is used to investigate the electric field intensity, the pulse number and the liquid-to-material ratio. The optimum extraction process of the acidic polysaccharide is obtained as follows: the electric field intensity is 18 kV/cm, the pulse number is 7 and the liquid-to-material ratio is 27 mL/g. At this time, the polysaccharide yield reaches a maximum value of 56.03 μg/mL. The crude polysaccharide is separated and purified by using DEAE-52 cellulose ion exchange column and Sephadex G-100 to obtain an acidic polysaccharide F1 with high purity. The molecular weight is 1.9895×105 Da. Its functional and structural characteristics need to be further studied.