Inhibition of growth and induction of apoptosis in human lung cancer cells by BR-OXPH

Abstract

The study was aimed at evaluating apoptotic potential of Br-oxph (4-bromo-N,N-diethyl-5,5-dimethyl-2,5-dihydro-1,2-oxaphosphol-2-amine 2-oxide) in vitro. The dose response effect of Br-oxph (dose range 1-3 mg/ml, for 48 h) on SK-MES-1 cells viability was determined by means of WST-1 cell proliferation assay. The half maximal inhibitory concentration (IC50) value was determined-1.8 mg/ml. The ability of the compound tested to induce apoptosis was tested by ELISA to detect cellular DNA fragmentation. We provided a quantitative assessment of the apoptotic potential of Broxph in human lung carcinoma cells at concentrations corresponding to IC50 and 2×IC50 for 3 hours. Treatment with 2×IC50 significantly increased the amount of cytoplasmic DNA-fragments. Results obtained from the present study confirm that Broxph target the cancerous cells towards apoptosis.