Species and number of bacterium may alternate IL-1β levels in the odontogenic cyst fluid

Abstract

Objectives: The role of oral bacteria in the etiopathogenesis of odontogenic cysts (OC) is controversial. Immune response is regulated by the cytokines secreted during infection. This study aims to describe the association in between bacteria and levels of cytokines in OC. Methods: Infected OC fluid samples were obtained from 25 odontogenic keratocysts and 14 radicular cysts (RC). Bacteria detection was performed by polymerase chain reaction on bacterial 16S rRNA genes. Cytokine levels in OC fluids were determined using "luminex" method. Results: Porphyromonas gingivalis was the most common bacteria in all samples (41.03%). Bacteria species number was higher in RCs. The significant difference was detected in terms of interleukine (IL)-1β levels to the number of bacteria contained in cyst fluids (p < 0.05). IL1-β level of cyst fluid group containing three or more species of bacteria increased compared with cyst fluid group containing two types of bacteria (p < 0.05). IL-1β level was high in cyst fluids with Campylobacter rectus and Treponema denticola or with three or more bacteria species. IL-1β level was higher in the cyst fluids with Enterococcus faecalis negative than E. faecalis positives. Conclusions: Our results suggest that species and the number of bacterium may differ IL-1β levels in the OC fluid.