Plant regeneration from somatic embryogenic suspension cultures of date palm

Abstract

Somatic embryogenesis is one of the most important technologies for plant regeneration of elite date palm cultivars. Recently, considerable progress has been made in the development and optimization of this technique from embryogenic cell suspension cultures. This chapter describes a procedure for the rapid development of a large number of somatic embryos from embryogenic cell suspension cultures. An efficient plant regeneration protocol via somatic embryogenesis from cell suspension cultures starting with shoot-tip explants to plantlet acclimatization also is fully described. Low concentrations of 6-benzylaminopurine (BAP) to 0.3 mg/L and high rate of subcultures each 7 days lead to improve the establishment and multiplication of somatic embryos in suspension cultures by limiting oxidative browning, associated with high total phenols and peroxidase activities. The detailed morphological observations have revealed the cells destined to become somatic embryos. Activated charcoal (AC) at 0.15 g/L has a positive effect on growth rate of somatic embryos by reducing tissue and medium browning, phenolics, and peroxidase activity.