The influence of divalent cations and chelators on aflatoxin B1 degradation by Flavobacterium aurantiacum

Abstract

The influence of divalent cations (Mg2+ and Ca2+) and chelators (EDTA and 1,10-phenanthroline) on aflatoxin B1 (AFB1) degradation by Flavobacterium aurantiacum was determined in an effort to elucidate the possible manner by which this organism degrades AFB1. AFB 1 (10 μg/ml) was added to 72-h cultures of F. aurantiacum that had been washed and resuspended in phosphate buffer (pH 7.0). High-performance liquid chromatography was used to determine AFB1 concentration in these cultures. Incubating cells with 0.1, 1, and 10 mM Ca2+ for 48 h significantly increased AFB1 degradation by 11.8, 13.5, and 14.0%, respectively, compared with F. aurantiacum cells alone. Likewise, incubation with 0.1, 1, and 10 mM Mg2+ for 48 h significantly increased AFB 1 degradation by 13.8, 13.3, and 13.1%, respectively. Incubating the bacterium with either divalent cation for 16 and 24 h did not significantly affect AFB1 degradation (P ≤ 0.05). Addition of 0.1, 1, and 10 mM EDTA and 0.1 and 1 mM 1,10-phenanthroline resulted in significant increases in AFB1 degradation after 24 h. Significantly less AFB1 degradation was observed using 10 mM 1,10-phenanthroline after 24-h incubation. These results suggest the involvement of Mg2+ and Ca2+ cations in AFB 1 degradation by F. aurantiacum.