Identification of a novel DRB1 allele, DRB1*1112, by sequence-based DRB typing

Abstract

We report here a novel DRB1 allele (DRB1*1112) identified during sequence-based HLA.DRB typing. Polymerase chain reaction with generic DRB primers and group-specific primers and subsequent sequencing yielded identical results. Molecular cloning and sequencing confirmed that the new DRB1 allele is identical to DRB1*11011 and 1129 at exon 2 except for a single nucleotide substitution at codon 37, changing the codon from Tyr (DRB1*11011) or Ser (DRB1*1129) to Phe..