MCP-1 gene polymorphisms in North Chinese patients with pulmonary tuberculosis

Abstract

Pulmonary tuberculosis (PTB) remains one of the most important infectious diseases worldwide. Several studies have suggested that genetic factors may affect the susceptibility to PTB, but the specifc genes involved have not been fully characterized. The gene for monocyte chemoattractant protein 1 (MCP-1) has been linked to an increased risk of tuberculosis in some Mexican and Korean populations. To explore the role of the MCP-1 gene in the susceptibility to PTB in a North Chinese population, we evaluated the association between MCP-1 -2518A/G gene polymorphisms and the risk for tuberculosis. Polymerase chain reaction amplifcation of genomic DNA followed by restriction fragment length polymorphism analysis was used. There was a signifcant increase in the frequency of the GG genotype of MCP-1 -2518 in 136 patients with PTB compared to that in 152 healthy controls (P = 0.008, X 2 = 7.133, odds ratio = 1.96). Similarly, the frequencies of the A/G alleles in the 2 groups differed; the frequency of allele G was higher in patients with PTB (P = 0.011, X 2 = 6.428, odds ratio = 1.536). In conclusion, the -2518A/G polymorphism in the MCP-1 gene was found to be associated with an increased susceptibility to PTB in a North Chinese population.