Synthesis of Protein-Directed Orange/Red-Emitting Copper Nanoclusters via Hydroxylamine Hydrochloride Reduction Approach and Their Applications on Hg 2 + Sensing

Abstract

Herein, we report a facile one-pot method for the synthesis of orange/red-emitting fluorescent copper nanoclusters (Cu NCs), in which bovine serum albumin (BSA) served as the capping scaffold (Cu NCs@BSA) and hydroxylamine hydrochloride (NH2OHHCl) as the reducing agent. To the best of our knowledge, this is the first time to adopt this mild reductant to synthesize fluorescent Cu NCs. The as-prepared Cu NCs@BSA exhibits strong orange or red fluorescence at room temperature depending on the synthesis process, and the maximum excitation and emission peaks were at 355nm and 615nm, 395nm and 645nm, respectively. Synthesis conditions including the amounts of NH2OHHCl, the selection of reducing agent, the molar ratio of BSA/Cu(NO3)2, the pH value, the reaction temperature, the reaction time, and various kinds of Cu sources have been systematically studied. Importantly, these Cu NCs exhibit excellent stability for at least 2 months when stored at 4°C in the dark, and they also show strong oxidation resistance toward H2O2. Moreover, the prepared Cu NCs have been successfully applied to sensitively and selectively sensing Hg2+ without suffering any interference from other metal ions and anions.