Ligand fishing is a convenient bioanalytical screening method that is based on the affinity selection of a ligand from a complex biological sample by an immobilized receptor. It is a versatile affinity-based screening approach and it has found application in multiple interacting pairs such as enzyme-inhibitor/activator, antigen-antibody, receptor-ligand, and protein-protein. Important parameters that affect the successful operation of the method are the high specificity and strong binding affinity of the interacting pair (e.g., enzyme-ligand complex) and the elution of the bound ligand from the complex. This chapter provides protocols for the synthesis of affinity adsorbent and its application in off-line ligand-fishing procedure for a 6His-tagged glutathione transferase (GST).
CITATION STYLE
Chronopoulou, E. G., Varotsou, C., Georgakis, N., Premetis, G., Ioannou, E., & Labrou, N. E. (2020). Ligand fishing: An approach for the discovery of inhibitors from complex biological mixtures. In Methods in Molecular Biology (Vol. 2089, pp. 235–243). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0163-1_15
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