A variety of designed RNA ring structures (ranging from triangles to hexagonal rings) have been reported in the scientific literature. Designing self-assembling RNA ring structures from structural motifs is, however, a nontrivial problem as there are many combinations of motifs and linking helices. Moreover, most combinations of motifs and linker helices will not lead to ring closure. A solution to this problem was recently published using a “design-by-catalog” approach where motif combinations that lead to rings are precomputed and tabulated. Here we present a web-browser based workflow for creating RNA rings using Galaxy, a web-based platform that can be used for workflow management. An example of how these RNA rings are generated and processed to create a 3D model of the ring is discussed.
CITATION STYLE
Sharan, R., Bindewald, E., Kasprzak, W. K., & Shapiro, B. A. (2017). Computational generation of RNA nanorings. In Methods in Molecular Biology (Vol. 1632, pp. 19–32). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7138-1_2
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