In situ profiling of the tumor-immune microenvironment (TiME) requires the ability to co-localize and detect multiple proteins simultaneously. Imaging mass cytometry (IMC), using the Hyperion™ imaging system is a novel multiplex imaging modality that currently enables detection of up to 50 markers on fixed tissues at subcellular resolution and thus has the potential to inform both pre-clinical and clinical research by providing investigators with spatially resolved information about the TiME. Here we provide an overview of the IMC workflow from sample fixation to analysis, with a focus on multiplex panel design and tissue staining.
CITATION STYLE
Cotechini, T., Jones, O., & Hindmarch, C. C. T. (2023). Imaging Mass Cytometry in Immuno-Oncology. In Methods in Molecular Biology (Vol. 2614, pp. 1–15). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2914-7_1
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