Polysome fractionation makes use of density gradients and ultracentrifugation to separate transcripts based on their specific number of bound ribosomes, and can be combined with downstream analysis such as cDNA-seq (commonly known as RNA-seq), microarray analysis, RT-qPCR, or Northern blotting. Here, we describe the application of Nanopore direct RNA sequencing to quantify monosome- and polysome-bound full-length transcripts after polysome fractionation, RNA cleanup, and size selection, using the yeast glucose stress response as an example use case.
CITATION STYLE
Nguyen, L. A. C., Inada, T., & Galipon, J. (2023). Nanopore Direct RNA Sequencing of Monosome- and Polysome-Bound RNA. Methods in Molecular Biology (Clifton, N.J.), 2632, 281–297. https://doi.org/10.1007/978-1-0716-2996-3_20
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