Separation and enrichment of hematopoietic stem cells for CCN studies

Abstract

The regulation of blood cell production (hematopoiesis) by CCN proteins is an area of increasing interest to hematologists. There is some discordance in the literature in this area due to the use of mixed or illdefi ned cell populations for experiments. Expression of, and response to, CCN proteins is specifi c to both cell type and differentiation status. Here, we describe methods to prepare defi ned hematopoietic cell populations and associated functional assays.