Biochemical tests and identification of potential indigenous bacteria from nickel post-mining land in Pomalaa

Abstract

The main problem in the nickel post-mining land in Pomalaa is the toxicity of nickel and chromium. The results of the analysis of soil samples showed that nickel levels reached 11103.74 mg/kg, and chromium reached 4030.17 mg/kg. The toxicity of nickel and chromium can cause the degradation of soil fertility. Bacteria are biological agents that are environmentally friendly, which can be used to reduce heavy metal toxicity and increase soil fertility on post-mining land. This study aims to examine biochemically six indigenous bacteria resistant to 10 ppm NiCl2 and four indigenous bacteria resistant to 10 ppm CrCl2 and identification. Based on the results of biochemical tests known indigenous bacteria from the genus Bacillus sp. is gram-positive bacteria that do not have the catalase enzyme, do not have the ability to use carbohydrates and produce endospores in their life cycle while indigenous bacteria from the genus Clostridium sp. is a gram-positive bacteria which in part has the enzyme catalase, all of which have the ability to use carbohydrates and only some of them produce endospores.