12-O-acetylphorbol-13-decanoate potently inhibits cytopathic effects of human immunodeficiency virus type 1 (HIV-1), without activation of protein kinase C

Abstract

Through bioactivity-guided fractionation, eight phorbol diesters, including five new ones (1-5), were isolated from the seeds of Croton tiglium collected in Egypt. 12-O-Acetylphorbol-13-decanoate (6) and 12-O- decanoylphorbol-13-(2-methylbutyrate) (4) potently inhibited the HIV-1- induced cytopathic effect on MT-4 cells (IC100 values of 7.6 ng/ml and 7.81 μg/ml, and CC0 values of 62.5 μg/ml and 31.3 μg/ml, respectively) without activating protein kinase C.