In vitro transcription of double-stranded DNA (dsDNA) induces a formation of two types of G-quadruplexes, intramolecular DNA G-quadruplexes or DNA:RNA hybrid G-quadruplexes, in clusters of guanine tracts. The formation of G-quadruplexes can be characterized by native PEG polyacrylamide gel electrophoresis, DMS footprinting, and ligand-induced photocleavage footprinting. The type of G-quadruplexes can be distinguished by transcription with 7-deaza-GTP (dzGTP) or 4-thio-uridine-5′-triphosphate (4SU) to detect the participation of RNA transcript.
CITATION STYLE
Zheng, K. wei, Zhang, J. yu, & Tan, Z. (2019). Characterization of Co-transcriptional Formation of G-Quadruplexes in Double-Stranded DNA. In Methods in Molecular Biology (Vol. 2035, pp. 243–255). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9666-7_14
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