Development of a Cost Effective Medium for Enhanced Production of Bacillus thuringiensis ?-endotoxin

  • Bhowmik A
  • Mourin M
  • Shishir M
  • et al.
N/ACitations
Citations of this article
12Readers
Mendeley users who have this article in their library.

Abstract

The present study was carried out to develop a sustainable production medium using locally available cheap raw materials for biopesticide production by Bacillus thuringiensis subsp. kurstaki (Btk) HD-73. In submerged fermentation (SmF) condition, the conventional Luria-Bertani (LB) medium which was enriched with nitrogen source (10% defatted soybean meal) supported 28.57% sporulation and 125% endotoxin increase over LB (alone). The effect of cystine on sporulation and endotoxin synthesis was highly pronounced in LB-soybean medium (LBS) with a range of 19.54% and 131.35% higher endotoxin yield respectively in SmF condition. While basal salts supplemented in soybean-cystine (SMc) medium, it resulted in 7.65% endotoxin yield compared to LB-soybean-cystine (LBSc) medium. Addition of molasses balanced the C: N ratio in the SMc medium thus helping 84.85% higher endotoxin synthesis after 24 hours fermentation. Substitution of basal salts with cost effective sea water yielded about 19.27% less endotoxin. The optimum medium thus obtained consisting of soybean extract-molasses-cystine with sea water was used in 3.0 L bioreactor cultivation for endotoxin synthesis by Btk HD-73 under 30% saturation of dO2 through cascade of agitation and aeration. The production rate obtained was 1.67 fold higher in bioreactor than in shake flask culture.Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 1-6

Cite

CITATION STYLE

APA

Bhowmik, A., Mourin, M., Shishir, M. A., Khan, S. N., & Hoq, M. M. (2016). Development of a Cost Effective Medium for Enhanced Production of Bacillus thuringiensis ?-endotoxin. Bangladesh Journal of Microbiology, 1–6. https://doi.org/10.3329/bjm.v32i0.28470

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free