MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.
CITATION STYLE
Pearson, J. A., & Wong, F. S. (2016). Identification of islet antigen-specific cd8 t cells using MHCI-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes. Methods in Molecular Biology, 1433, 119–125. https://doi.org/10.1007/7651_2015_295
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