Functional approaches for studying embryonic development have greatly advanced thanks to the CRISPR-Cas9 gene editing technique. Previously practiced in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian Phallusia mammillata.
CITATION STYLE
McDougall, A., Hebras, C., Gomes, I., & Dumollard, R. (2021). Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation. In Methods in Molecular Biology (Vol. 2219, pp. 217–230). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0974-3_13
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