Evaluation of the cytotoxic effect of rutin prenanoemulsion in lung and colon cancer cell lines

Abstract

In this work, prenanoemulsion of rutin was prepared using PEG and Tween as emulsifiers via homogenization and evaporation techniques. The particle size of rutin was investigated with high-resolution transmission electron microscopy (HR-TEM); particle size distribution and its chemical structure were analysed by nuclear magnetic resonance (NMR) and Fourier transformed infrared (FT-IR) spectroscopy. It was found that rutin in the prenanoemulsion has excellent solubility in water with the size approximately 15 nm. The chemical structure of nanorutin in prenanoemulsion was identical to that of the pure rutin. It suggested that there is no chemical modification of rutin in the prenanoemulsion. From high-performance liquid chromatography (HPLC), the amount of rutin in the prenanoemulsion was determined to be 9.27%. The cytotoxic effect of rutin in the preemulsion was investigated via in vitro tests to determine rutin’s efficacy in A549 lung cancer cell and colon cancer cell treatment. The results demonstrated that rutin in the prenanoemulsion could inhibit A549 lung cancer cells and colon cancer cells efficiently.