DNA Extraction from Seeds

Abstract

The extraction of DNA from seeds is a very important step in every molecular study involving plant genetics. Seeds possesses many compounds that effectively interfere with DNA extraction and subsequently with downstream procedures. Despite the importance of this subject, little attention is given to improving and standardizing the current protocols. Thus, each study has to adapt and develop the best protocol modifications adjusted to its own specific requirements. After discussing several biotechnical difficulties and different solutions for each one, we propose a widely used protocol and diverse possibilities of protocol changes displayed in a diagram to ease every possible change and adjustment.