In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches as well as high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.
CITATION STYLE
Applewhite, D. A., Christine, C. A., Griffis, E. R., & Quintero, O. A. (2016). Imaging of the cytoskeleton using live and fixed drosophila tissue culture cells. In Methods in Molecular Biology (Vol. 1365). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3124-8_4
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