To investigate the feasibility of using fresh water from Mangueira Lagoon (Rio Grande do Sul, Brazil) for biomass production in open raceway ponds (0.7 m long, 0.18 m wide, 0.075 m deep) we studied the influence of nutrient addition (carbon as sodium bicarbonate, nitrogen as urea, phosphate, sulfate, ferric iron, magnesium and potassium) on the growth rate of the cyanobacteria Spirulina platensis using a 22 factorial design. In unsupplemented lagoon water production of S. platensis was 0.78 ± 0.01 g/1 (dry weight basis) while the addition of 2.88 g/1 of sodium bicarbonate (without added urea, phosphate, sulfate or metal ions) resulted in 0.82 ± 0.01 g/1 after 400 hours of culture. The further addition of phosphate and metal ions resulted in growth for up to 750 h and a final S. platensis biomass of 1.23 ± 0.04 to 1.34 ± 0.03 g/1.
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Costa, J. A. V., Colla, L. M., & Filho, P. D. (2003). Spirulina platensis growth in open raceway ponds using fresh water supplemented with carbon, nitrogen and metal ions. Zeitschrift Fur Naturforschung - Section C Journal of Biosciences, 58(1–2), 76–80. https://doi.org/10.1515/znc-2003-1-214