Visualization of anticancer salmonella typhimurium engineered for remote control of therapeutic proteins

Abstract

Tumor-targeting bacteria are studied for their ability to carry therapeutic molecules to tumors or, when designed as imaging probes, to visualize the infection pathway. The present protocol describes a method to achieve remote control of therapeutic gene expression in bacteria which are also engineered to visualize the therapeutic process. This strategy may increase the safety of bacteria used to deliver therapeutic genes to tumors in vivo.