Significant efforts have sought to uncover the protein profile of Arabidopsis mitochondria to act as a model for the mitochondrial proteome from plants. A combination of techniques have been undertaken to achieve this goal. We outline a basic two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation of mitochondrial proteins, in-gel trypsination techniques, complex protein lysate digestions, and the identification of proteins by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry.
CITATION STYLE
Heazlewood, J. L., & Millar, A. H. (2007). Arabidopsis mitochondrial proteomics. Methods in Molecular Biology (Clifton, N.J.), 372, 559–571. https://doi.org/10.1007/978-1-59745-365-3_38
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