Protein-ligand interactions

Abstract

This chapter will review the use of analytical ultracentrifugation for measuring protein-ligand interactions. This class of interactions is important in biology, biomedicine and industry and includes a diverse range of ligands from small molecules, such as drugs and enzyme substrates, through to large macromolecular complexes, e.g. lipid emulsions. Given its experimental diversity and sample flexibility, the analytical ultracentrifuge is a premier platform for detecting and quantitating protein-ligand interactions. This will be discussed herein by reviewing recent studies focused on protein-drug, enzyme-substrate and apolipoprotein-lipid interactions.