Differential heat shock tolerance and expression of heat-inducible proteins in two stored-product psocids

Abstract

The recent recognition of psocids as a major concern in stored products and also the reemergence of heat treatment as a control tactic of stored-product insects led to the present investigation. The objectives of this study were to determine whether there are differences in heat shock tolerance of two species of stored-product psocids-Lepinotus reticulatus Enderlein (Trogiidae) and Liposcelis entomophila (Enderlein) (Liposcelididae)-and to determine whether heat shock proteins (HSPs) underlay such tolerance. Time-response bioassays were therefore carried out at increasing temperatures for both psocids. The lethal time (LT)50 and LT95 estimates were correlated with the expression of heat shock proteins after exposure at the same range of temperatures for 30min. The expression of HSP was determined through Western blot analyses using HSP 70 antibody. Liposcelis entomophila was more than two-fold more tolerant than L. reticulatus for nearly all of the range of temperatures (≥40.0°C). Expression of HSP 70 was not observed for either of the psocid species, but the expression of two low-molecular-mass heat-inducible proteins (HIPs; 23 and 27 kDa) was observed in L. entomophila. The expression of these small proteins was induced by exposure to higher temperatures, and the trend was particularly strong for HIP 27. In contrast, no expression of small heat-inducible proteins was detected in L. reticulatus, reflecting its higher susceptibility to heat treatments. The relatively high heat tolerance of L. entomophila might help explain its more common occurrence in grain stored in warmer regions of the world.