Determination of intraparticle immobilized enzyme distribution under moderate diffusion conditions

Abstract

The method Presented earlier by Hossain and Do determine the active enzyme distribution and relevant rate parameters under the condition of strong diffusional resistance is extended in this article to cover the cases of comparable diffusion and reaction rates (3 < ϕ < 20). The theory proposed herein is tested wtih the experimental data of hydrogen perioxide‐catalase immobilized on controlled‐pore glass (CPG) particles of small size (150 μm). © 1992 John Wiley & Sons, Inc. Copyright © 1992 John Wiley & Sons, Inc.