Employing the DNA microarray technique, we previously reported the alteration in gene expression of nucleocytoplasmic transport factors, importins and exportins, induced by 1,25-dihydroxyvitamin D3 (DVD) in human leukemia HL-60 cells. Here, we used the quantitative reverse transcription-polymerase chain reaction method to confirm such previous findings, and compared them with those from the cells treated with all-trans-retinoic acid (ATRA). The results indicated that the gene expression of the transport factors examined was mostly down-regulated following differentiation induced by DVD and ATRA, but importin α5 gene expression was up-regulated in either case. The differences were found in the gene expression of importin α3 and exportin 6 between the cells after treatments with DVD and ATRA. These variations may be related to the difference between HL-60 cell lineages differentiating into monocytes/macrophages and granulocytes. The present findings provide further evidence to support the important roles of importins and exportins in cell differentiation.
CITATION STYLE
Suzuki, T., Ishigami, Y., Okada, N., Kaneko, A., Fukutomi, R., & Isemura, M. (2008). Differentiation-associated alteration in gene expression of importins and exportins in human leukemia HL-60 cells. Biomedical Research, 29(3), 141–145. https://doi.org/10.2220/biomedres.29.141
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