Polyclonal antibodies against the human cell surface CD34 marker

Abstract

A highly efficient and inexpensive laboratory method of production and purification of polyclonal antibodies against the human cell surface CD34 marker was developed. It was demonstrated that unglycosy- lated recombinant protein cloned in E. coli cells and containing the extracellular fragment of the human CD34 antigen maintained the necessary antigenic determinants during isolation from bacteria and during immunization, induced the production of specific polyclonal antibodies, which could recognize the native antigen on the cell surface. The obtained antibodies can be used for CD34+ cell phenotyping by the immu- nocytochemistry and flow cytometry methods. © Allerton Press, Inc., 2011.