Numerous methods for the measurement of tacrolimus and cyclosporine A involving traditional liquid chromatography tandem mass spectrometry (LC-MS/MS) have previously been described. The majority of these methods use solid-phase extraction, liquid-liquid extraction, or protein precipitation extraction with instrument run times greater than 15 s per sample. Continued demands in clinical labs for greater efficiency and throughput have put increased stress on traditional technologies such as high-performance liquid chromatography-ultraviolet detection (HPLC-UV) and traditional LC-MS/MS. As an improvement to the existing methods, we describe a sensitive ultrafast LC-MS/MS with run times of less than 15 s per sample.
CITATION STYLE
Bjergum, M. W., Jannetto, P. J., & Langman, L. J. (2019). Simultaneous determination of tacrolimus and cyclosporine a in whole blood by ultrafast LC-MS/MS. In Methods in Molecular Biology (Vol. 1872, pp. 111–118). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8823-5_11
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