Extracellular vesicles (EVs) including exosomes and microvesicles are nanometer-sized vesicles released by cells to deliver lipids, cellular proteins, mRNAs, and noncoding RNAs, thereby facilitating intercellular communication without direct cell-to-cell contacts. Due to their nanoscale size, EVs have been visualized under microscopy in vitro. We here describe a strategy to label EVs with Gaussia luciferase for noninvasive bioluminescence imaging and monitoring of systemically administered EVs in vivo. © 2014 Springer Science+Business Media New York.
CITATION STYLE
Lai, C. P., Tannous, B. A., & Breakefield, X. O. (2014). Noninvasive in vivo monitoring of extracellular vesicles. Methods in Molecular Biology, 1098, 249–258. https://doi.org/10.1007/978-1-62703-718-1_19
Mendeley helps you to discover research relevant for your work.