Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents

  • Ward W
N/ACitations
Citations of this article
9Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The isolation of mammalian sperm heads from their tails is complicated by the relatively high density of the tails, but facilitated by the fact that protamine condensation of the sperm chromatin and the insolubility of the perinuclear theca make the sperm nucleus stable in sodium dodecyl sulfate. Two methods are described for the isolation of rodent sperm nuclei using sucrose step gradients in which the sperm nuclei are only centrifuged one time, minimizing potential damage by mechanical stress.

Cite

CITATION STYLE

APA

Ward, W. S. (2013). Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents (pp. 437–444). https://doi.org/10.1007/978-1-62703-038-0_37

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free