The hepatocyte nuclear factor 3β stimulates the transcription of the human insulin-like growth factor I gene in a direct and indirect manner

37Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Promoter 1 (P1) of the human insulin-like growth factor I (IGF-I) gene is most active in adult liver. In this study we show that HNF-3β, a member of the winged helix protein family of liver-enriched transcription factors, has a strong stimulatory effect on the activity of P1. Transient transfection experiments in combination with bandshift and DNase I footprinting analysis revealed the presence of two HNF-3 binding sites in the proximal promoter region of P1. Both binding sites, which are well conserved in evolution, are required for maximal transactivation. Studies employing HNF-3 mutant constructs indicated that IGF-I expression is also regulated indirectly by HNF-3β as a consequence of enhanced expression of HNF-1α. This liver- enriched transcription factor has previously been shown to transactivate P1. Thus, HNF-3β regulates the expression of the human IGF-I gene via two distinct mechanisms.

Cite

CITATION STYLE

APA

Nolten, L. A., Steenbergh, P. H., & Sussenbach, J. S. (1996). The hepatocyte nuclear factor 3β stimulates the transcription of the human insulin-like growth factor I gene in a direct and indirect manner. Journal of Biological Chemistry, 271(50), 31846–31854. https://doi.org/10.1074/jbc.271.50.31846

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free