N-glycosylation is a maturation event necessary for the correct function, efficiency, and stability of a high number of biopharmaceuticals. This chapter presented here proposes various methods to determine whether, how, and where a plant pharmaceutical is N-glycosylated. These methods rely on blot detection with glycan-specific probes, specific deglycosylation of glycoproteins followed by mass spectrometry, N-glycan profile analysis, and glycopeptide identification by LC-MS. © 2009 Humana Press.
CITATION STYLE
Bardor, M., Cabrera, G., Stadlmann, J., Lerouge, P., Cremata, J. A., Gomord, V., & Fitchette, A. C. (2009). N-glycosylation of plant recombinant pharmaceuticals. Methods in Molecular Biology, 483, 239–264. https://doi.org/10.1007/978-1-59745-407-0_14
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