Isolation and culture properties of a thermophilic agarase-producing strain, Microbulbifer sp. SD-1

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Abstract

An agar-degrading enzyme-producing strain was isolated from seawater. The isolate was identified as Microbulbifer sp. SD-1 by 16S rRNA sequencing analysis. The optimal pH and temperature for growth were 6.0 and 30°C, respectively, and growth was possible at pH 9.0 and 60°C. The isolate required 5% NaCl for optimal growth and showed 45% growth activity without NaCl. Agar concentrations of 0-0.4% in the medium did not affect growth. Thin-layer chromatography analysis revealed that this strain could degrade agar into a monosaccharide and oligosaccharide, which may have industrial applications. © The Korean Society of Fisheries and Aquatic Science.

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Kim, D. K., Jang, Y. R., Kim, K. H., Lee, M. N., Kim, A. R., Jo, E. J., … Lee, E. W. (2011). Isolation and culture properties of a thermophilic agarase-producing strain, Microbulbifer sp. SD-1. Fisheries and Aquatic Sciences, 14(3), 186–191. https://doi.org/10.5657/FAS.2011.0186

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