The Selection of DNA Aptamers Against the Fc Region of Human IgG

Abstract

Aptamers can bind with various kinds of target molecules and therefore they have highly potential for using in the therapeutic, diagnostic, biosensing, purification applications, etc. The aptamer that could specifically bind to the Fc region of antibody is very attractive to the applications of biosensors or diagnostics. Herein, a RNA aptamer specific to the Fc region of human IgG was adopted to convert to a DNA sequence as the template for producing mutated sequences for selection. Computational approaches were applied in the selection processes and then the evaluation results were verified by the ELISA assay. Finally, four new DNA aptamers were discovered and confirmed their binding ability to the Fc region of human IgG. According to experimental results, these DNA aptamers could not generate the binding amounts of antibodies as much as the original RNA sequence. However, these DNA aptamers still have potential to be used for the immobilization of human IgG in the applications of diagnostics or biosensors.